Methodology Used for NLEA Analysis
Fat. AOAC 16th Edition (1995), 32.1.14 is the general method. Use of specialized modifications or different types of extractions apply to certain other categories of foods.
Protein. AOAC 16th Edition (1995), 32.1.22 (factor to convert % nitrogen to % protein may vary from product to product).
Moisture. Typical methods are AOAC 16th Edition (1995), 32.1.02 and 32.1.03. Drying times and temperatures, the use of vacuum and other conditions will vary for different sample classes.
Ash. AOAC 16th Edition (1995), 32.1.05.
Total Carbohydrates. Calculation of total carbohydrates as the difference between 100% less % moisture, less % protein, less % ash, and less % fat.
Total Dietary Fiber. AOAC 16th Edition (1995), 45.4.07 (enzymatic-gravimetric method).
Sugars Profile. AOAC 16th Edition (1995), 32.2.07 (liquid chromatographic method).
Cholesterol. AOAC 16th Edition (1995), 41.1.45.
Fatty Acid Profile. Extraction of fat and fatty substances, followed by AOAC 16th Edition (1995), 41.1.28.
Vitamin A. In-house liquid chromatographic methods for both retinol-type compounds (animal derived) and carotenes (plant derived).
Vitamin C. (Ascorbic Acid) AOAC 16th Edition (1995), 45.1.14 (titrimetric method). In-house liquid chromatographic method used when titrimetry unreliable.
Sodium, Calcium, Iron (+Potassium, when Requested). Ashing (AOAC method 32.1.05, above), followed by Atomic Absorption Spectrophotometry.
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